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drug h89  (Tocris)


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    Tocris drug h89
    Drug H89, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/drug+h89/H+89+dihydrochloride/pm41903111-159-13-15
    Average 95 stars, based on 148 article reviews
    drug h89 - by Bioz Stars, 2026-09
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    Article Title: Mechanisms of norepinephrine induced Ca 2+ responses in perivascular adipose tissue.
    Article Snippet: .. Blocking PKA with the 306 Downloaded from journals.physiology.org/journal/ajpendo (2402:0800:6343:DF27:7D60:CF16:1511:DB5B) on March 31, 2026. drug H89 (Tocris, 2910, 10μM) increased responses in mPVAT by 21% (p=0.0365, mean=0.6324 ± 0.6647 307 SD, n=91 cells, 4 mice) (Fig. 4B’), but had no effect on adipocyte responses in aPVAT and WAT (aPVAT, 308 p=0.6473, mean=0.5022 ± 0.4123 SD, n=90 cells, 4 mice; WAT, p=0.1682, mean=0.5979 ± 0.3838 SD, 309 n=49 cells, 4 mice) (Fig. 4B’’-4B’’’). ..



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    drug h89  (Tocris)
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    Drug H89, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chemical compound, drug h89  (Selleck Chemicals)
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    ( A ) Diagram showing the schedule of Tamoxifen (TAM) and <t>H89</t> administration. ( B ) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. ( C ) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. ( D ) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. ( E ) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. ( F ) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1 CreERT ; Bmpr1a f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in ( C, D, and G ). p<0.05 was considered as statistically significant. Figure 7—source data 1. Original file for the western blot in .
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    ( A ) Diagram showing the schedule of Tamoxifen (TAM) and <t>H89</t> administration. ( B ) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. ( C ) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. ( D ) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. ( E ) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. ( F ) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1 CreERT ; Bmpr1a f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in ( C, D, and G ). p<0.05 was considered as statistically significant. Figure 7—source data 1. Original file for the western blot in .
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    chemical compound, drug h89  (Beyotime)
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    Hc-CATH downregulates AXL via inhibiting COX-2/PGE 2 /AC/PKA pathway. A , effect of Hc-CATH on COX-2 protein level. A549 cells were incubated with Hc-CATH (1.25, 2.5, or 5 μM) or PBS (solvent of Hc-CATH) at 37 °C for 24 h, COX-2 protein level was examined by Western blot ( upper panel ) and analyzed by ImageJ ( lower panel ). B , effect of Hc-CATH on the enzymatic activity of COX-2. Hc-CATH (1.25, 2.5, 5, and 10 μM) or PBS was incubated with COX-2. After incubation at 37 °C for 10 min, the enzymatic activity of COX-2 was detected by enzyme activity inhibitor screening kit. Celecoxib (COX-2 inhibitor, 100 nM) was used as positive control. C , effect of Hc-CATH on PGE 2 production. A549 cells were incubated with Hc-CATH (5 μM) at 37 °C. After incubation for 24 h, the level of PGE 2 in the cell supernatant was detected by ELISA. D and E , effect of AC ( D ) and PKA ( E ) on Hc-CATH-induced AXL downregulation. A549 cells were incubated with forskolin (FSK, agonist of AC, 10 μM) or <t>H89</t> (inhibitor of PKA, 5 μM) at 37 °C. After incubation for 1 h, the culture media were removed, cells were washed three times with PBS, and fresh culture media were added to cells in the presence of Hc-CATH (5 μM). After incubation for 24 h, AXL protein level was examined by Western blot ( upper panel of D and E ) and analyzed by ImageJ ( lower panel of D and E ). ns, not significant, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. AC, adenylate cyclase; COX-2, cyclo-oxygenase-2; Hc-CATH, a cathelicidin antimicrobial peptide identified from the sea snake Hydrophis cyanocinctus ; PGE 2 , prostaglandin E 2.
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    drug h89 tocris  (Tocris)
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    Tocris drug h89 tocris
    Figure 4. Increased PP2A activity maintains tetrodotoxin (TTX)-induced Shank3 hypophosphorylation. (A) Diagram showing the potential roles of kinases and phosphatases in regulating activity-dependent Shank3 phosphorylation. (B) Representative Western blot showing the impacts of inhibiting CAMKII (KN62, KN93) or PKA <t>(H89)</t> on Shank3 phosphorylation at baseline and upon TTX treatment. (C) Quantification of S1615 phosphorylation in (B) (two-way ANOVA with post-hoc Tukey’s test: DMSO vs. KN62, p>0.9999, DMSO vs. KN93, p=0.8148, DMSO vs. H89, p=0.9112, DMSO vs. picrotoxin (PTX), *p=0.0406, PTX vs. PTX/KN62, **p=0.0040, PTX vs. PTX/KN93, ****p<0.0001, PTX vs. PTX/H89, ****p<0.0001, n = 5 biological replicates). Dashed line indicates the DMSO control. (D) Quantification of PP2A activity after 1 hr TTX treatment (Un, n = 5, TTX, n = 5; paired t-test: **p=0.0018). (E) Quantification of PP2A activity after 24 hr TTX treatment (Un, n = 7, TTX, n = 7; paired t-test: *p=0.0129). (F, G) Western blot analyses showing changes in S1615 phosphorylation after 1 hr (F) or 24 hr (G) TTX treatment, with inhibition of PP2A by okadaic acid (OKA, 50 nM) during the
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    drug h89 selleck  (Selleck Chemicals)
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    Selleck Chemicals drug h89 selleck
    Figure 7. ACE2 pathway induces a thermogenesis program through the Akt signaling and the PKA signaling. Primary brown adipocytes were isolated, cultured, and treated with Ang-(1-7) (10–6 M) for 24 hr, Akt inhibitor MK2206 (20 μM) for 24 hr, PKA inhibitor <t>H89</t> (30 μM) for 24 hr, or adenylylcyclase inhibitor SQ-22536 (10 μM) for 24 hours. (A, B) Representative western blots showing the changes of p-Akt308 and Akt in BAT of Ace2-/y (A) and Leprdb/
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    ( A ) Diagram showing the schedule of Tamoxifen (TAM) and H89 administration. ( B ) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. ( C ) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. ( D ) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. ( E ) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. ( F ) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1 CreERT ; Bmpr1a f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in ( C, D, and G ). p<0.05 was considered as statistically significant. Figure 7—source data 1. Original file for the western blot in .

    Journal: eLife

    Article Title: BMP signaling maintains auricular chondrocyte identity and prevents microtia development by inhibiting protein kinase A

    doi: 10.7554/eLife.91883

    Figure Lengend Snippet: ( A ) Diagram showing the schedule of Tamoxifen (TAM) and H89 administration. ( B ) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. ( C ) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. ( D ) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. ( E ) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. ( F ) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1 CreERT ; Bmpr1a f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in ( C, D, and G ). p<0.05 was considered as statistically significant. Figure 7—source data 1. Original file for the western blot in .

    Article Snippet: Chemical compound, drug , H89 , Selleck , S1582 , 10 mg/kg.

    Techniques: Immunostaining, Mutagenesis, Control, Quantitation Assay, Staining, Activity Assay, Expressing, Marker, Western Blot

    Journal: eLife

    Article Title: BMP signaling maintains auricular chondrocyte identity and prevents microtia development by inhibiting protein kinase A

    doi: 10.7554/eLife.91883

    Figure Lengend Snippet:

    Article Snippet: Chemical compound, drug , H89 , Selleck , S1582 , 10 mg/kg.

    Techniques: In Situ

    Hc-CATH downregulates AXL via inhibiting COX-2/PGE 2 /AC/PKA pathway. A , effect of Hc-CATH on COX-2 protein level. A549 cells were incubated with Hc-CATH (1.25, 2.5, or 5 μM) or PBS (solvent of Hc-CATH) at 37 °C for 24 h, COX-2 protein level was examined by Western blot ( upper panel ) and analyzed by ImageJ ( lower panel ). B , effect of Hc-CATH on the enzymatic activity of COX-2. Hc-CATH (1.25, 2.5, 5, and 10 μM) or PBS was incubated with COX-2. After incubation at 37 °C for 10 min, the enzymatic activity of COX-2 was detected by enzyme activity inhibitor screening kit. Celecoxib (COX-2 inhibitor, 100 nM) was used as positive control. C , effect of Hc-CATH on PGE 2 production. A549 cells were incubated with Hc-CATH (5 μM) at 37 °C. After incubation for 24 h, the level of PGE 2 in the cell supernatant was detected by ELISA. D and E , effect of AC ( D ) and PKA ( E ) on Hc-CATH-induced AXL downregulation. A549 cells were incubated with forskolin (FSK, agonist of AC, 10 μM) or H89 (inhibitor of PKA, 5 μM) at 37 °C. After incubation for 1 h, the culture media were removed, cells were washed three times with PBS, and fresh culture media were added to cells in the presence of Hc-CATH (5 μM). After incubation for 24 h, AXL protein level was examined by Western blot ( upper panel of D and E ) and analyzed by ImageJ ( lower panel of D and E ). ns, not significant, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. AC, adenylate cyclase; COX-2, cyclo-oxygenase-2; Hc-CATH, a cathelicidin antimicrobial peptide identified from the sea snake Hydrophis cyanocinctus ; PGE 2 , prostaglandin E 2.

    Journal: The Journal of Biological Chemistry

    Article Title: A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor

    doi: 10.1016/j.jbc.2022.102471

    Figure Lengend Snippet: Hc-CATH downregulates AXL via inhibiting COX-2/PGE 2 /AC/PKA pathway. A , effect of Hc-CATH on COX-2 protein level. A549 cells were incubated with Hc-CATH (1.25, 2.5, or 5 μM) or PBS (solvent of Hc-CATH) at 37 °C for 24 h, COX-2 protein level was examined by Western blot ( upper panel ) and analyzed by ImageJ ( lower panel ). B , effect of Hc-CATH on the enzymatic activity of COX-2. Hc-CATH (1.25, 2.5, 5, and 10 μM) or PBS was incubated with COX-2. After incubation at 37 °C for 10 min, the enzymatic activity of COX-2 was detected by enzyme activity inhibitor screening kit. Celecoxib (COX-2 inhibitor, 100 nM) was used as positive control. C , effect of Hc-CATH on PGE 2 production. A549 cells were incubated with Hc-CATH (5 μM) at 37 °C. After incubation for 24 h, the level of PGE 2 in the cell supernatant was detected by ELISA. D and E , effect of AC ( D ) and PKA ( E ) on Hc-CATH-induced AXL downregulation. A549 cells were incubated with forskolin (FSK, agonist of AC, 10 μM) or H89 (inhibitor of PKA, 5 μM) at 37 °C. After incubation for 1 h, the culture media were removed, cells were washed three times with PBS, and fresh culture media were added to cells in the presence of Hc-CATH (5 μM). After incubation for 24 h, AXL protein level was examined by Western blot ( upper panel of D and E ) and analyzed by ImageJ ( lower panel of D and E ). ns, not significant, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. AC, adenylate cyclase; COX-2, cyclo-oxygenase-2; Hc-CATH, a cathelicidin antimicrobial peptide identified from the sea snake Hydrophis cyanocinctus ; PGE 2 , prostaglandin E 2.

    Article Snippet: Chemical compound, drug , H89 , Beyotime Biotechnology , Catalog no.: S1643 , .

    Techniques: Incubation, Solvent, Western Blot, Activity Assay, Positive Control, Enzyme-linked Immunosorbent Assay

    Journal: The Journal of Biological Chemistry

    Article Title: A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor

    doi: 10.1016/j.jbc.2022.102471

    Figure Lengend Snippet:

    Article Snippet: Chemical compound, drug , H89 , Beyotime Biotechnology , Catalog no.: S1643 , .

    Techniques: Flow Cytometry, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    Figure 4. Increased PP2A activity maintains tetrodotoxin (TTX)-induced Shank3 hypophosphorylation. (A) Diagram showing the potential roles of kinases and phosphatases in regulating activity-dependent Shank3 phosphorylation. (B) Representative Western blot showing the impacts of inhibiting CAMKII (KN62, KN93) or PKA (H89) on Shank3 phosphorylation at baseline and upon TTX treatment. (C) Quantification of S1615 phosphorylation in (B) (two-way ANOVA with post-hoc Tukey’s test: DMSO vs. KN62, p>0.9999, DMSO vs. KN93, p=0.8148, DMSO vs. H89, p=0.9112, DMSO vs. picrotoxin (PTX), *p=0.0406, PTX vs. PTX/KN62, **p=0.0040, PTX vs. PTX/KN93, ****p<0.0001, PTX vs. PTX/H89, ****p<0.0001, n = 5 biological replicates). Dashed line indicates the DMSO control. (D) Quantification of PP2A activity after 1 hr TTX treatment (Un, n = 5, TTX, n = 5; paired t-test: **p=0.0018). (E) Quantification of PP2A activity after 24 hr TTX treatment (Un, n = 7, TTX, n = 7; paired t-test: *p=0.0129). (F, G) Western blot analyses showing changes in S1615 phosphorylation after 1 hr (F) or 24 hr (G) TTX treatment, with inhibition of PP2A by okadaic acid (OKA, 50 nM) during the

    Journal: eLife

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling

    doi: 10.7554/elife.74277

    Figure Lengend Snippet: Figure 4. Increased PP2A activity maintains tetrodotoxin (TTX)-induced Shank3 hypophosphorylation. (A) Diagram showing the potential roles of kinases and phosphatases in regulating activity-dependent Shank3 phosphorylation. (B) Representative Western blot showing the impacts of inhibiting CAMKII (KN62, KN93) or PKA (H89) on Shank3 phosphorylation at baseline and upon TTX treatment. (C) Quantification of S1615 phosphorylation in (B) (two-way ANOVA with post-hoc Tukey’s test: DMSO vs. KN62, p>0.9999, DMSO vs. KN93, p=0.8148, DMSO vs. H89, p=0.9112, DMSO vs. picrotoxin (PTX), *p=0.0406, PTX vs. PTX/KN62, **p=0.0040, PTX vs. PTX/KN93, ****p<0.0001, PTX vs. PTX/H89, ****p<0.0001, n = 5 biological replicates). Dashed line indicates the DMSO control. (D) Quantification of PP2A activity after 1 hr TTX treatment (Un, n = 5, TTX, n = 5; paired t-test: **p=0.0018). (E) Quantification of PP2A activity after 24 hr TTX treatment (Un, n = 7, TTX, n = 7; paired t-test: *p=0.0129). (F, G) Western blot analyses showing changes in S1615 phosphorylation after 1 hr (F) or 24 hr (G) TTX treatment, with inhibition of PP2A by okadaic acid (OKA, 50 nM) during the

    Article Snippet: DOI: https://doi.org/10.7554/eLife.74277 19 of 31 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pAAV- CMV- PI- EGFP- WPRE- bGH Gift from James M. Wilson Addgene# 105530; RRID:Addgene_105530 Commercial assay or kit Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668- 027 Commercial assay or kit Gibson Assembly Master Mix New England Biolabs Cat# E2611S Commercial assay or kit Lambda protein phosphatase New England Biolabs Cat# P0753S Commercial assay or kit BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Commercial assay or kit Protein- G Magnetic Beads Thermo Fisher Scientific Cat# 88847 Commercial assay or kit SimplyBlue SafeStain Thermo Fisher Scientific Cat# LC6060 Commercial assay or kit PP2A Immunoprecipitation Phosphatase Assay Kit Millipore Cat# 17- 313 Commercial assay or kit Ni- NTA Superflow Agarose Beads QIAGEN Cat# 30410 Chemical compound, drug Tetrodotoxin Tocris Cat# 1069 Chemical compound, drug Bicuculline methobromide Tocris Cat# 0109 Chemical compound, drug Picrotoxin Sigma- Aldrich Cat# P1675 Chemical compound, drug Okadaic acid Santa Cruz Cat# sc- 3513 Chemical compound, drug Tautomycetin Tocris Cat# 2305 Chemical compound, drug Fostriecin Tocris Cat# 1840 Chemical compound, drug KN62 Tocris Cat# 1277 Chemical compound, drug KN93 Tocris Cat# 1278 Chemical compound, drug H89 Tocris Cat# 2910 Chemical compound, drug Sequencing- grade trypsin Promega Cat# V5111 Chemical compound, drug Tandem Mass Tag (TMT) 10plex Thermo Fisher Scientific Cat# 90110 Software, algorithm Image Lab Software Bio- Rad RRID:SCR_014210 https://www.bio-rad.com/en-us/product/imagelab-software?ID=KRE6P5E8Z&source_wt= imagelabsoftware_surl Software, algorithm ZEN Black Zeiss RRID:SCR_018163 https://www.zeiss.com Software, algorithm Metamorph Molecular Devices RRID:SCR_002368 http://www.moleculardevices.com/Products/Software/ Meta-Imaging-Series/MetaMorph.html Software, algorithm Fiji Fiji RRID:SCR_002285 http://fiji.sc Software, algorithm GraphPad Prism GraphPad RRID:SCR_002798 http://www.graphpad.com/ Software, algorithm IGOR pro Wavemetrics RRID:SCR_000325 https://www.wavemetrics. com/products/igorpro/igorpro.htm Software, algorithm Spectrum mill v.7.00.208 Agilent Technologies Software, algorithm R v 4.0 The R Foundation RRID:SCR_001905 https://www.R-project.org/ Continued Continued on next page Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Techniques: Activity Assay, Phospho-proteomics, Western Blot, Control, Inhibition

    Figure 7. ACE2 pathway induces a thermogenesis program through the Akt signaling and the PKA signaling. Primary brown adipocytes were isolated, cultured, and treated with Ang-(1-7) (10–6 M) for 24 hr, Akt inhibitor MK2206 (20 μM) for 24 hr, PKA inhibitor H89 (30 μM) for 24 hr, or adenylylcyclase inhibitor SQ-22536 (10 μM) for 24 hours. (A, B) Representative western blots showing the changes of p-Akt308 and Akt in BAT of Ace2-/y (A) and Leprdb/

    Journal: eLife

    Article Title: ACE2 pathway regulates thermogenesis and energy metabolism

    doi: 10.7554/elife.72266

    Figure Lengend Snippet: Figure 7. ACE2 pathway induces a thermogenesis program through the Akt signaling and the PKA signaling. Primary brown adipocytes were isolated, cultured, and treated with Ang-(1-7) (10–6 M) for 24 hr, Akt inhibitor MK2206 (20 μM) for 24 hr, PKA inhibitor H89 (30 μM) for 24 hr, or adenylylcyclase inhibitor SQ-22536 (10 μM) for 24 hours. (A, B) Representative western blots showing the changes of p-Akt308 and Akt in BAT of Ace2-/y (A) and Leprdb/

    Article Snippet: DOI: https://doi.org/10.7554/eLife.72266 15 of 21 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug FCCP Sigma- Aldrich C2920 Chemical compound, drug Oligomycin A Sigma- Aldrich 75351–5 MG Chemical compound, drug Rotenone Sigma- Aldrich R8875- 1G Chemical compound, drug MK2206 Selleck 1032350- 13- 2 Chemical compound, drug H89 Selleck 130964- 39- 5 Chemical compound, drug SQ- 22536 Selleck 17318- 31- 9 Other Chow, 60% HFD Research Diets D12492 Antibody Anti- UCP1 (rabbit polyclonal) Abcam #10983 RRID: AB_2241462 (1:1000) Antibody Anti- PGC1ɑ (rabbit polyclonal) Abcam #54,481 RRID: AB_881987 (1:1000) Antibody Anti- OXPHOS Abcam #110413 RRID: AB_2629281 (1:1000) Antibody Anti- Mas1 (rabbit polyclonal) Alomone #AAR- 013 RRID: AB_2039972 (1:1000) Antibody Anti- Akt (rabbit polyclonal) Cell signaling #9272 RRID: AB_329827 (1:1000) Antibody Anti- p- Akt308 (rabbit monoclonal) Cell signaling #13038 RRID: AB_2629447 (1:1000) Antibody Anti- PKA (rabbit polyclonal) Cell signaling #4782 RRID: AB_2170170 (1:1000) Antibody Anti- p- PKA (rabbit polyclonal) Cell signaling #9,621 RRID: AB_330304 (1:1000) Antibody Anti- ACE2 (rabbit monoclonal) Cell signaling #92,485 (1:1000) Antibody Actin (rabbit monoclonal) Cell signaling #4,970 RRID: AB_2223172 (1:1000) Sequence- based reagent Cidea_F Invitrogen RT- qPCR primer TCCT ATGC TGCA CAGA TGACG Sequence- based reagent Cidea_R This paper RT- qPCR primer TGCT CTTC TGTA TCGC CCAGT Sequence- based reagent Ppargc1a_F This paper RT- qPCR primer GCAC CAGA AAAC AGCT CCAAG Sequence- based reagent Ppargc1a_R This paper RT- qPCR primer CGTC AAAC ACAG CTTG ACAGC Sequence- based reagent Ucp1_F This paper RT- qPCR primer TCTC AGCC GGCT TAAT GACTG Sequence- based reagent Ucp1_R This paper RT- qPCR primer GGCT TGCA TTCT GACC TTCAC Sequence- based reagent Prdm16_F This paper RT- qPCR primer ACAC GCCA GTTC TCCA ACCTGT Continued on next page Continued Cao, Shi, Zhang et al. eLife 2022;10:e72266.

    Techniques: Isolation, Cell Culture, Western Blot